Overexpression of Glutathione S-Transferase α in Clear Cell Renal Cell Carcinoma

Autor:	Ximing J. Yang, Min-Han Tan, Kim L. Wang, Maria Tretiakova, Veronica Papavero, Bin Tean Teh, Jun Sugimura, Shang Tian Chuang, Fan Lin, Peiguo Chu
Rok vydání:	2005
Předmět:	Kidney Cell General Medicine Biology urologic and male genital diseases medicine.disease Clear cell renal cell carcinoma Glutathione S-transferase medicine.anatomical_structure Renal cell carcinoma Complementary DNA Clear cell carcinoma Cancer research medicine biology.protein Clear cell
Zdroj:	American Journal of Clinical Pathology. 123:421-429
ISSN:	1943-7722 0002-9173
DOI:	10.1309/aqxr6b2qpugd638c
Popis:	To determine its diagnostic value, we evaluated glutathione S-transferase α (GST-α) expression in a large number of renal cell carcinomas (RCCs). GST-α messenger RNA (mRNA) levels from 70 renal neoplasms were analyzed with complementary DNA (cDNA) microarray chips containing 21,632 cDNA clones. Furthermore, 348 primary renal tumors and 24 metastatic RCCs were subjected to immunohisto-chemical analysis with a GST-α–specific antibody. GST-α mRNA was elevated significantly (11.4-fold) in a majority of clear cell RCCs (28/43 [65.1%]; 28/39 [71.8%] with adjustments for informative spots) compared with other kidney tumors (1/27 [3.7%]). Strong and diffuse GST-α immunoreactivity was demonstrated in a majority of clear cell (166/202 [82.2%]; mean intensity, 2.41) and metastatic clear cell RCCs (17/24 [70.8%]; mean intensity, 2.62). Other renal tumor types did not exhibit significant GST-α immunoreactivity, confirming mRNA results. Through cDNA microarrays and immunohistochemical analysis, we demonstrated GST-α as a biomarker for clear cell RCCs.
Databáze:	OpenAIRE
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