Agrobacterium-Mediated Transformation of Hieracium aurantiacum

Autor:	N. K. Borst, Ross Bicknell
Rok vydání:	1994
Předmět:	Kanamycin Resistance biology Agrobacterium fungi food and beverages Kanamycin Plant Science biology.organism_classification Transformation (genetics) chemistry.chemical_compound chemistry Seedling Apomixis Botany medicine Gene Ecology Evolution Behavior and Systematics DNA medicine.drug
Zdroj:	International Journal of Plant Sciences. 155:467-470
ISSN:	1537-5315 1058-5893
Popis:	An Agrobacterium-mediated transformation system, based on the neomycin phosphotransferase II (NPTII) gene, is described for the apomictic plant Hieracium aurantiacum. Integration of forcign DNA was confirmed by Southern analysis and its expression demonstrated by assaying for NPTII enzymatic activity. NPTII activity, which confers kanamycin resistance, was inherited by clonal seedlings of transformed plants. Growth of untransformed seedlings was inhibited by 20 mg/L kanamycin, while transformants continued to grow at 300 mg/L. The clarity of the response, together with the convenience of screening at the seedling stage, makes kanamycin resistance a valuable tool for quantifying reproductive mechanisms in facultatively apomictic plants of this species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::438e5b1fdf81c06a54910f8b73cc2d39 https://doi.org/10.1086/297184 Zobrazit plný text záznamu Plný text ve formátu PDF