A cyclohexadepsipeptide from entomogenous fungi Metarhizium anisopliae inhibits the Helicobacter pylori induced pathogenesis through attenuation of vacuolating cytotoxin-A activity
| Autor: | David T.W. Tzeng, Yew-Min Tzeng, Shih-Hsien Weng, Chih Ho Lai, Chun-Jung Lin, Yerra Koteswara Rao, Jau-Jin Liu, Min-Chuan Kao, Tzu-Li Lu, Yu-Wen Hsieh |
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| Rok vydání: | 2015 |
| Předmět: |
Metarhizium anisopliae
Bioengineering Vacuole Biology Helicobacter pylori bacterial infections and mycoses biology.organism_classification Applied Microbiology and Biotechnology Biochemistry digestive system diseases Microbiology Pathogenesis medicine.anatomical_structure Vacuolization Gastric mucosa medicine V-ATPase Bacteria |
| Zdroj: | Process Biochemistry. 50:134-139 |
| ISSN: | 1359-5113 |
| DOI: | 10.1016/j.procbio.2014.10.017 |
| Popis: | Infection with Helicobacter pylori in the gastric mucosa is thought to be increased the risk of peptic ulcer disease and gastric cancer. Vacuolating cytotoxin A (VacA) was an important bacterial virulence factor and was closely associated with H. pylori-induced pathogenesis. Upon H. pylori infection, the VacA can be transferred directly from bacteria into the host cells where can stimulate vacuolar-type ATPase (V-ATPase) proton pump and induce the formation of vacuoles in gastric epithelial cells followed by intoxication of cells. Destruxin B (Dtx-B), a cyclodepsipeptide from the fungus Metarhizium anisopliae bearing insecticidal and anticancer effects. In this study, Dtx-B demonstrated inhibitory effects against H. pylori VacA-induced vacuolization in gastric epithelial cells. Our data further showed that attenuation of H. pylori-induced vacuolization in gastric epithelial cells by Dtx-B is mediated through inhibition of V-ATPase activity rather than VacA expression levels. The results from this study reveal a novel role for Dtx-B in the modulation of H. pylori VacA-induced pathogenesis. |
| Databáze: | OpenAIRE |
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