Stimulation of Xenopus laevis Oocyte Maturation by Methyl-β-Cyclodextrin1
| Autor: | Susan E. Sadler, Nicole D. Jacobs |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Germinal vesicle biology Cholesterol Xenopus Cell Biology General Medicine Oocyte biology.organism_classification Cell biology chemistry.chemical_compound medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry Internal medicine medicine Signal transduction Protein kinase A Gametogenesis Hormone |
| Zdroj: | Biology of Reproduction. 70:1685-1692 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.103.026161 |
| Popis: | The cholesterol-depleting drug methyl-b-cyclodextrin (Me-bCD) was tested for its effects on amphibian oocyte maturation, cholesterol depletion, and low-density membrane recovery. Progesterone-induced oocyte maturation was accelerated by pretreatment of cells with 5‐50 mM Me-b-CD in a dose-dependent manner. Treatment of oocytes with 50 mM Me-b-CD alone was sufficient to induce germinal vesicle breakdown, stimulate formation of meiotic spindles, and stimulate phosphorylation of mitogen-activated protein kinase over time courses longer than those observed after progesterone treatment. After short-term (30 min) labeling of oocytes with [ 3 H]cholesterol, 30‐90 min of treatment with 5‐50 mM Me-b-CD removed 50%‐70% of cellassociated label, and cholesterol depletion was not observed with a-cyclodextrin. After long-term (20‐23 h) labeling of oocytes with [ 3 H]cholesterol, Me-b-CD treatment resulted in dosedependent cholesterol depletion in the 5‐50 mM range, and 50 mM Me-b-CD removed approximately 50% of cell-associated label after 9 h. Treatment of oocytes with 5‐50 mM Me-b-CD also decreased recovery of low-density membrane by detergentfree sucrose gradient centrifugation. These results implicate cholesterol and low-density membrane domains in the signaling mechanisms leading to germinal vesicle breakdown in amphibian oocytes. gametogenesis, meiosis, progesterone, signal transduction, steroid hormones |
| Databáze: | OpenAIRE |
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