Prebleaching of softwood and hardwood pulps by a high performance xylanase belonging to a novel clade of glycosyl hydrolase family 11

Autor:	David L. Blum, Alexander Varvak, Brad A. Lowery, Martin Kazaoka, Jack O. Turner, Thomas Woodward, Ali R. Esteghlalian, David P. Weiner, Geoffrey P. Hazlewood, Bill Hancock
Rok vydání:	2008
Předmět:	Softwood Pulp (paper) Bioengineering engineering.material Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound chemistry Kraft process Hydrolase Hardwood Xylanase engineering Glycosyl Food science Clade Biotechnology
Zdroj:	Enzyme and Microbial Technology. 42:395-403
ISSN:	0141-0229
Popis:	To discover and develop a xylanase enzyme that can significantly enhance wood pulp bleachability under typical kraft process conditions (high temperature and alkaline pH), close to 200 xylanases from diverse environments were discovered and screened using a 3-stage (XDEp) pulp biobleaching assay. Four xylanases belonging to a new clade of glycosyl hydrolase family 11 outperformed all other enzymes by reducing bleaching chemical consumption by 15–25% on softwood and hardwood pulps. During the screening process, many thermostable and alkali-tolerant family 10 xylanases were also identified, however, family 10 xylanases in general were not as effective as those from family 11 in enhancing wood pulp bleachability. The candidate selected for developing a prebleaching xylanase enzyme product, Xyn11a, retained 80% of its maximum activity after incubation at 65 °C for 30 min.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::435e5b4eeacd15b48f8bf55040a27e3f https://doi.org/10.1016/j.enzmictec.2007.12.004 Zobrazit plný text záznamu Full Text from ScienceDirect