Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis
| Autor: | Paula V. Bennett, Denise C. Monteleone, John Trunk, Betsy M. Sutherland, John C. Sutherland |
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| Rok vydání: | 2001 |
| Předmět: |
Gel electrophoresis
Materials science Fluorophore Gel electrophoresis of nucleic acids DNA damage Clinical Biochemistry Analytical chemistry food and beverages Biochemistry Analytical Chemistry chemistry.chemical_compound chemistry Electronic imaging Agarose gel electrophoresis Pulsed-field gel electrophoresis Biophysics DNA |
| Zdroj: | ELECTROPHORESIS. 22:843-854 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/1522-2683()22:5<843::aid-elps843>3.0.co;2-9 |
| Popis: | DNA damages that can be converted to single- or double strand breaks can be quantified by separating DNA by gel electrophoresis and obtaining a quantitative image of the resulting distribution of DNA in the gel. We review the theory of this method and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to acquire images of fluorophore labeled DNA. |
| Databáze: | OpenAIRE |
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