Bacterial Community of Koumiss from Mongolia Investigated by Culture and Culture-Independent Methods

Autor: Eva Marie Breines, Wilhelm H. Holzapfel, Pen Ren, Armin Kousha, Sigmund Sperstad, Gerd Jorun Yttergård, Zhigang Zhou, Renate Andersen, Einar Ringø, Ann Kristin Andreassen, Heidi Mathilde Johansen, Kirstin Opsal, Ellinor Hareide, Jacques Godfroid
Rok vydání: 2014
Předmět:
Zdroj: Food Biotechnology. 28:333-353
ISSN: 1532-4249
0890-5436
DOI: 10.1080/08905436.2014.964253
Popis: The bacterial community of fermented horse milk (koumiss) from Mongolia was studied using three methods: cultivation, direct identification by 16S rRNA clone library and denaturing gradient gel electrophoresis (DGGE). Ninety-eight strains were isolated by traditional cultivation and 61 of those were randomly selected for further identification by 16S rRNA gene sequencing. The strains were dominated by lactic acid bacteria (LAB; six different lactobacilli), Acinetobacter, Bacillus and Psychrobacter. Construction of the clone library analysis revealed that 16S sequences of 220 clones, genus Lactobacillus was dominant, but Streptococcus thermophilus, Acetobacter pasteurianus and uncultured clones were also detected. Ten unique bands were sequenced from the DGGE and revealed: Lactococcus lactis, Lactococcus lactis subsp. lactis, Clostridium acidurici, Acinetobacter johnsonii, Dickeya sp., Enterobacter sp., Pseudomonas sp., Raoultella sp., and Ruminococcus sp.. In vitro growth inhibition of three human pathoge...
Databáze: OpenAIRE
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