UVA1 irradiation induces deoxyribonuclease dependent apoptosis in cutaneousT-cell lymphoma in vivo

Autor: Gregor von Kobyletzki, Peter Altmeyer, C Pieck, Hans Georg Mannherz, Klaus Hoffmann, Markus Stücker, Oliver Heine, Harald Stephan
Rok vydání: 2000
Předmět:
Zdroj: Photodermatology, Photoimmunology & Photomedicine. 16:271-277
ISSN: 0905-4383
DOI: 10.1034/j.1600-0781.2000.160607.x
Popis: Cutaneous T-cell lymphoma (CTCL) is a malignancy of mature T-cells, predominantly of the helper phenotype, that primarily invade the skin. Different photo- and chemotherapeutic treatments are known to be beneficial in early-stage CTCL. This observation has initiated prospective investigations into the efficacy of phototherapeutic regimens. The purpose of our study was to investigate the ability of medium-dose UVA1 phototherapy (60 J/cm2) to induce apoptosis (programmed cell death) in skin infiltrating T-cells of CTCL in vivo. We describe the results of three different staining methods for formalin-fixed, paraffin-embedded tissue sections. The in situ end-labeling (ISEL) procedure, nuclear staining using the DNA-binding fluorochrome Hoechst 33342, and immunohistochemistry using polyclonal antibodies against recombinant mouse deoxyribonuclease I (DNase I) demonstrated that UVA1 irradiation was able to induce marked apoptosis in CTCL. Thereby, ISEL and Hoechst staining clearly revealed DNA-condensation and nuclear fragmentation, accompanied by the formation of typical "apoptotic bodies". The accumulation of DNase I immunoreactivity in the cytoplasm of lymphocytes in UVA1 irradiated skin indicated that DNase I or DNase I-related endonucleases may have acted as apoptotic endonuclease(s) which were synthesized after UVA1 irradiation prior to their apoptotic elimination.
Databáze: OpenAIRE
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