PENGARUH WAKTU INKUBASI PADA PROSES SEXING SPERMA BERBASIS GLUTATHIONE TERHADAP MOTILITAS DAN MEMBRAN PLASMA UTUH CHILLED SEMEN DOMBA LOKAL

Autor:	Nurcholidah Solihati, Nena Hilmia, Annisaa Yusrina
Rok vydání:	2018
Předmět:	chemistry.chemical_compound Animal science chemistry Semen Sexing Glutathione Sperm Experimental research Sperm motility Completely randomized design Incubation period
Zdroj:	Jurnal Ilmu Ternak Universitas Padjadjaran. 18:45
ISSN:	2621-5144 1410-5659
DOI:	10.24198/jit.v18i1.17307
Popis:	The aim of this study was to examine the effect of incubation time on sexing process based on glutathione to the motility and Intact Plasma Membrane (IPM) of chilled ram semen. The separation of X and Y sperm conducted by using BSA method. This research was experimental research using completely randomized design (CRD) with three treatment namely, 45 minutes (T1), 60 minutes (T2) and 75 minutes (T3). Data were analyzed using Anova followed by Duncan's multiple range test. Material used in this research was the fresh semen from ram with sperm motility ≥ 70% and 5 mM of glutathione. The result of this research showed that the highest percentage of motility in upper and bottom fraction belong to T1 (69,7% dan 68,8%), followed by T2 (66,4% dan 64,5%) and T3 (57,9% dan 57,6%). In addition, the result of this research that the highest percentage of IPM in upper and bottom fraction belong to T1(75% dan 71,3%), followed by T2 (69,9% dan 68,4%) and T3 (66,9% dan 65,5%). It can be concluded that the incubation time of 45 minute is the optimum time on sexing process based on glutathione so that the motility and IPM of chilled ram semen can be maintained.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::433a9516af96ba87a9bd608fdb205e12 https://doi.org/10.24198/jit.v18i1.17307 Zobrazit plný text záznamu