| Popis: |
Procedures are presented for the continuous-flow, simultaneous measurement of concentration and radioactivity of purein metabolites separated by high-performance liquid chromatography (HPLC). A microprocessor-controlled radioactivity flow detector connected in series to a UV-flow detector provides on-line quantitative monitoring of separated components in the post-column effluent stream. Through use of two HPLC separations —reversed-phase and anion-exhange— quantitation of all major purine nucleotides, nucleosides and bases is possible. The procedures provide a rapid, sensitive, and convenient means for the systematic study of purine metabolism. |
