Human TAF-Iα promotes oncogenic transformation via enhancement of cell proliferation and suppression of apoptosis

Autor: Larisa V Kozikova, Natalya A Shcherbatova, Stanislav A Antonov, Lyudmila E Andreeva, E. V. Novosadova, Nella V Khaidarova, Anastasia R Narsullaeva, Valentina V Nenasheva, Lyudmila A Sleptsova, Ekaterina A Polteva, Vyacheslav Z Tarantul, Ekaterina A Stepanenko, Irina V. Makarova
Rok vydání: 2021
Předmět:
Zdroj: In Vitro Cellular & Developmental Biology - Animal. 57:531-538
ISSN: 1543-706X
1071-2690
DOI: 10.1007/s11626-021-00572-8
Popis: Template activating factor-I (TAF-I) is a multifunctional protein involved in various biological processes including the inhibition of histone acetylation, DNA replication, cell cycle regulation, and oncogenesis. Two main TAF-I isoforms with different N-termini, TAF-Iα and TAF-Iβ (SET), are expressed in cells. There are numerous data about functional properties of TAF-Iβ, whereas the effects of TAF-Iα remain largely unexplored. Here, we employed focus formation and cell proliferation assays, TUNEL staining, cytological analysis, and RT-qPCR to compare the effects of human TAF-Iα and TAF-Iβ genes, transiently expressed in Rat2 cells and in Misgurnus fossilis loaches. We found that both TAF-I isoforms possessed equal oncogenic potential in these systems. Furthermore, an overexpression of human TAF-Iα and TAF-Iβ in Rat2 cells promoted their proliferation. Accordingly, the mitotic index was increased in the transgenic loaches expressing human TAF-Iα or TAF-Iβ. TUNEL assay as well as downregulation of p53 gene and upregulation of bcl-2 gene in these transgenic loaches demonstrated that both isoforms suppressed apoptosis. Thus, TAF-Iα isoform exerts the same oncogenic potential as TAF-Iβ, likely by suppressing the apoptosis and promoting cell proliferation.
Databáze: OpenAIRE
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