Detection of Heterobasidion annosum s. l. [(Fr.) Bref.] in Norway Spruce by Polymerase Chain Reaction

Autor:	O. Wienhaus, H. Sandermann, C. Langebartels, G. Bahnweg, Evelyn M. Möller, S. Anegg
Rok vydání:	2002
Předmět:	biology Armillaria Physiology fungi Heterobasidion annosum Picea abies Plant Science biology.organism_classification law.invention law Botany Multiplex polymerase chain reaction Genetics Internal transcribed spacer Heterobasidion Agronomy and Crop Science Polymerase chain reaction Repeat unit
Zdroj:	Journal of Phytopathology. 150:382-389
ISSN:	1439-0434 0931-1785
Popis:	Internal transcribed spacer (ITS) sequences of the rDNA repeat unit of Heterobasidion annosum were used to design specific primers for the detection and quantification of this important forest pathogen by polymerase chain reaction (PCR). Specificity of detection was cross-checked against a variety of other fungi (saprophytes, root pathogens, mycorrhizal fungi) which may occur in the same environment. As little as 1 pg fungal DNA (equiv. to 10-40 genomes) could be detected in 200 ng spruce root DNA (from 1 mg fresh spruce root). The Heterobasidion-specific primers allowed simultaneous detection of Armillaria spp. in multiplex PCR. The method was successfully applied to increment cores of Norway spruce from the forest region Tharandter Wald (Saxonia, Germany), Oberbarenburg (East Ore Mountains, Saxonia) and Oberschleissheim (north of Munich, Bavaria).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::43278e3adf02e5b7a1a91494d7740218 https://doi.org/10.1046/j.1439-0434.2002.00772.x Zobrazit plný text záznamu Plný text ve formátu PDF