Factors affecting rooting ofin vitroshoots of walnut cultivars
| Autor: | Yuan Lichai, Wang Qingming, Gu Ruisheng, Pei Dong |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | The Journal of Horticultural Science and Biotechnology. 82:223-226 |
| ISSN: | 2380-4084 1462-0316 |
| DOI: | 10.1080/14620316.2007.11512223 |
| Popis: | SummaryFactors affecting the rooting of in vitro shoots were investigated using precocious walnut (Juglans regia L.) cv. Xinzaofeng. In vitro sub-culture was the most important step to achieve rooting of the shoots. Rooting of shoots was markedly affected by the method of induction, 3-indole-butyric acid (IBA) concentrations, light conditions, as well as shoot quality. Rooting rates of ≥ 75.4% were obtained with an optimised rooting protocol in which vigorous shoots were sub-cultured 45-times at 20 d intervals. These shoots appeared tender, with small leaves. A transfer method of root induction was applied and shoots were first induced on a semi-solidified, diluted Walnut Specific Medium (1/4 DKW) with 25 µM IBA in the dark for 12 d at 25° ± 3°C, then cultured on semi-solidified 1/4 DKW medium plus vermiculite, without IBA, with a 16 h photoperiod (53 µmol m–2 s–1). This protocol was extended to five other walnut cultivars, and rooting rates of ≥ 60.5% were achieved in all cases. |
| Databáze: | OpenAIRE |
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