| Autor: |
Aqeel A. Fatmi, Gregory V. Williams |
| Rok vydání: |
1987 |
| Předmět: |
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| Zdroj: |
Journal of Liquid Chromatography. 10:2461-2472 |
| ISSN: |
0148-3919 |
| DOI: |
10.1080/01483918708068927 |
| Popis: |
A reversed-phase, high performance liquid chromatographic method is developed and validated for the simultaneous determination of acetaminophen and hydrocodone bitartrate in pharmaceutical dosage forms. The method employs a Radialpak Cyanopropylsilane Cartridge with a mobile phase containing water, acetonitrile and dibutylamine phosphate (90:10:1). The detection is carried out using a variable wavelength UV-detector set at 215 nm. The acetaminophen showed linearity from 2.0 to 8.0 mg/mL range with a correlation coefficient of 0.999. The hydrocodone bitartrate showed linearity from 0.020 to 0.080 mg/mL range with a correlation coefficient of 0.999. Recoveries for acetaminophen averaged about 100.2% and hydrocodone bitartrate about 102.0% with a standard deviation of about 2.0%. System suitability showed excellent resolution of major peaks. The method is suitable for composite, content uniformity and dissolution assay of capsules and tablets. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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