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Figure S1. ULBP1 staining in a tissue microarray containing 60 glioblastoma tissues and 10 normal tissues, scale bar = 250 μm. Figure S2. ULBP3 staining in a tissue microarray containing 60 glioblastoma tissues and 10 normal tissues, scale bar = 250 μm. Figure S3. The cell-surface expression of CD3 in the indicated cells was analyzed by flow cytometry. The RAJI cell line was used as a negative control. Figure S4. The morphology of the suspended cell spheres formed in serum-free neural stem cell medium composed of DMEM/F12, 20 ng/ml EGF, 20 ng/ml bFGF, and 1x B27. Figure S5. The levels of the indicated cytokines were assessed by ELISA. T cells were incubated with GSC-3# cells at an E:T ratio of 5:1. The results are presented as the mean volume ± SD, ***, P < 0.001; ns, not significant. Figure S6. NKG2D-BBz CAR-T cells lysed U-87MG cells effectively in mice. (A) B-NDG mice were injected with 1 × 106 stable luciferase transfected U-87MG cells subcutaneously and imaged 7 days prior to T cell infusion. After mice received T cells treatment, photographs were taken serially at indicated time. (B) Comparison of tumor bioluminescent signal among the indicated groups at different time points. Figure S7. Persistence of NKG2D-BBz CAR-T cells in mice. B-NDG mice were injected with 1 × 106 stable luciferase transfected U-87MG cells subcutaneously and received T cells treatment 7 days later. Then human genomic DNA in blood was detected using qPCR at indicated time. Figure S8. Growth curves for the indicated cells. The CAR-T cells were counted every 2 days. The data are presented as the mean ± SD; ns, not significant. (DOCX 3450 kb) |
