High Developmental Competence of Pig Oocytes after Meiotic Inhibition with a Specific M-Phase Promoting Factor Kinase Inhibitor, Butyrolactone I1
| Autor: | Billy N. Day, Qing-Yuan Sun, Brad A. Didion, Randall S. Prather, Liangxue Lai, Guangming Wu, Kwang-Wook Park, Jiude Mao, Tod C. McCauley |
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| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Biology of Reproduction. 67:170-177 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | Butyrolactone I specifically inhibits M-phase promoting factor activation and prevents the resumption of meiosis. These experiments were conducted to examine effects of butyrolactone I on pig oocytes in a serum-free maturation system. The first experiment was conducted to determine the effect of butyrolactone I (0-100 microM) on nuclear maturation. At concentrations of > or =12.5 microM, germinal vesicle breakdown was prevented in >90% of the oocytes after 24 h of culture. In the second experiment, the kinetics of in vitro maturation of butyrolactone I-treated oocytes was investigated. Oocytes were treated with 0 or 12.5 microM butyrolactone I and FSH for 20 h and then cultured with LH in the absence of butyrolactone I for another 24 h. Fewer butyrolactone I-treated oocytes reached MII stage at 36 h compared with controls (5.8% vs. 62.4%, P 0.05) were found between controls and treated groups in cleavage rate, blastocyst rate, or mean number of cells per blastocyst. Effects of butyrolactone I on mitogen-activated protein kinase activation and localization of microfilaments and active mitochondria were examined by Western blot analysis and laser scanning confocal microscopy, respectively. The results suggested that although butyrolactone I reversibly inhibited germinal vesicle breakdown and mitogen-activated protein kinase activation, it did not affect mitochondrial and microfilament dynamics. Butyrolactone I is a potent inhibitor of nuclear maturation of porcine oocytes, and the inhibition is fully reversible. |
| Databáze: | OpenAIRE |
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