Development of Allotriploid Embryos from Female Olive Flounder Paralichthys olivaceus Crossed with Male Starry Flounder Platichthys stellatus
| Autor: | 정효선 ( Hyo Sun Jung ), 고민균 ( Min Gyun Ko ), 김동수 ( Dong Soo Kim ), 이효빈 ( Hyo Bin Lee ) |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Paralichthys biology Hatching Embryogenesis Zoology Embryo 04 agricultural and veterinary sciences biology.organism_classification Platichthys Olive flounder Fishery 03 medical and health sciences 030104 developmental biology embryonic structures 040102 fisheries 0401 agriculture forestry and fisheries Ploidy Starry flounder |
| Zdroj: | Korean Journal of Fisheries and Aquatic Sciences. 49:628-634 |
| ISSN: | 0374-8111 |
| DOI: | 10.5657/kfas.2016.0628 |
| Popis: | We investigated the characteristics and rate of development of allotriploid embryos derived from a cross between female olive flounder Paralichthys olivaceus and male starry flounder Platichthys stellatus. The allotriploidy was induced by cold shocking fertilized eggs three minutes post-fertilization at 3°C for 45 minutes. The average cellular DNA content of the allotriploid embryos was 2.06±0.03 pg/cell, which is equal to the sum of the cellular DNA content of a diploid olive flounder (1.42 pg/cell) and a haploid starry flounder (0.66 pg/haploid cell). The first cleavage, mid-blastula, gastrula and Kupffer`s vesicle appearance stages of the allotriploid eggs began at 1.5, 8, 13 and 26 hours after cold shocking at 18°C, respectively. The developmental rate of allotriploid eggs was equivalent to that of diploid and triploid olive flounder eggs at 10, 14 and 18°C. However, the hatching times of allotriploid eggs, 7 h at 10°C, 5 h at 14°C and 4 h at 18°C, were earlier than those of diploid and triploid olive flounder. |
| Databáze: | OpenAIRE |
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