Silver nanoparticles and silver salt (AgNO3) elicits morphogenic and biochemical variations in callus cultures of sugarcane
Autor: | Aamir Ali, Hamid Rashid, Rashid Iqbal, Abdul Rauf, Naveed Iqbal Raja, Mubashir Hussain, Muhammad Iqbal, Seema Hassan Satti, Umair A. Khan, Najma Shaheen, Muhammad Ejaz, Hafiza Saira |
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Rok vydání: | 2019 |
Předmět: |
chemistry.chemical_classification
Reactive oxygen species biology Chemistry Metal ions in aqueous solution 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology medicine.disease_cause Malondialdehyde 01 natural sciences Silver nanoparticle 0104 chemical sciences Electronic Optical and Magnetic Materials Superoxide dismutase chemistry.chemical_compound Catalase Callus biology.protein medicine Electrical and Electronic Engineering 0210 nano-technology Oxidative stress Biotechnology Nuclear chemistry |
Zdroj: | IET Nanobiotechnology. 13:896-904 |
ISSN: | 1751-875X 1751-8741 |
Popis: | The research work was arranged to check the role of AgNPs and silver ions on callus cells of sugarcane (Saccharum spp. cv CP-77,400). AgNPs were synthesized chemically and characterized by UV-Vis spectra, XRD and SEM. AgNPs and silver ions were applied in various concentrations (0, 20, 40, 60 ppm) to sugarcane calli and the induced stress was characterized by studying various morphological and biochemical parameters. AgNPs and silver ions treatments produced high levels of malondialdehyde, proline, proteins, TP and TF contents. Similarly, CAT, SOD and POX activity was also significant in both treatments. The lower concentration of AgNPs and silver ions (20 ppm) provided maximum intracellular GSH level. This work mainly showed effects of AgNPs and silver ions on sugarcane calli in terms of morphological aberrations and cell membrane damage due to severe oxidative stress and production of enhanced levels of enzymatic and non-enzymatic antioxidants as self-defence to tolerate oxidative stress by scavenging reactive oxygen species. These preliminary findings will provide the way to study ecotoxicity mechanism of the metal ions and NPs in medicine industry and in vitro toxicity research. Furthermore, silver ions alone and their chemically synthesised AgNPs can be used for various biomedical applications in future. |
Databáze: | OpenAIRE |
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