High-performance transmission electron microscopy of cryospecimens
| Autor: | D. Hoffmeister, U. B. Hezel, E. Zellmann |
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| Rok vydání: | 1984 |
| Předmět: | |
| Zdroj: | Proceedings, annual meeting, Electron Microscopy Society of America. 42:276-277 |
| ISSN: | 2690-1315 0424-8201 |
| DOI: | 10.1017/s0424820100111495 |
| Popis: | Chemical fixation, resin embedding and subsequent staining with heavy metals can both produce artefacts and limit the resolution in the electron microscopic specimen /1-5/. The objective is thus to observe the specimens in the electron microscope in the frozen-hydrated state, the one most similar to the native state.All preparation steps such as cryofixation, cryosectioning and cryotransfer to the cryo-transmission electron microscope (CryoTEM) should be performed below 145K /5/ in order to observe the specimen in a matrix of vitrified ice avoiding any crystallization artefacts. In the CryoTEM itself the temperature of the frozen-hydrated specimen should be kept much lower to avoid devitrification caused by the electron beam /1/. - To meet all these requirements a special cryotransfer system and cryostage for the Zeiss transmission electron microscopes have been developed /7/. |
| Databáze: | OpenAIRE |
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