Nano- und Chromobodies für high-content und super-resolution imaging

Autor:	Ulrich Rothbauer, Bettina-Maria Keller, Julia Maier, Björn Tränkle, Sören Segan
Rok vydání:	2018
Předmět:	0301 basic medicine Fluorophore Materials science Cellular imaging Pharmacology toxicology Nanotechnology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Microscopy Nano Molecular Biology Biotechnology
Zdroj:	BIOspektrum. 24:274-277
ISSN:	1868-6249 0947-0867
Popis:	Nano- and chromobodies are recombinant binding molecules that have expanded the possibilities of advanced cellular imaging. Here we provide an overview how chromobodies can be implemented to visualize localization and dynamics of endogenous proteins in live-cell high-content imaging. Additionally, we highlight recent advances in the development of nanobodies to label cellular structures with high densities and minimal fluorophore displacement for super-resolution microscopy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::42f61001fb8a5b8e9c634ea968eea2a2 https://doi.org/10.1007/s12268-018-0919-4 Zobrazit plný text záznamu Full text from SpringerLink