AAV-driven expression of channelrhodopsin-2 in lingual myofibers enables light activation of lingual electromyogram activity

Autor: Michele Singer, Ethan Benevides, Sabhya Rana, Robert Martinez, Brian Barral, Michael Sunshine, Barry Byrne, David Fuller
Rok vydání: 2023
Předmět:
Zdroj: Physiology. 38
ISSN: 1548-9221
1548-9213
DOI: 10.1152/physiol.2023.38.s1.5726047
Popis: Contraction of the tongue muscles is needed for effective swallowing, speaking and breathing. Thus, inadequate tongue muscle activation can contribute to devastating conditions such as dysarthria, dysphagia, and obstructive sleep apnea. Existing treatments for these clinical conditions are often ineffective and/or poorly tolerated. Optogenetic technology allows for exceptional temporal and spatial precision for stimulating cells and may therefore provide a tool activating the tongue muscles to promote airway patency or enhance swallow function. The purpose of the current study was to test the hypothesis that lingual injection of an AAV9 vector encoding channelrhodopsin-2 (ChR2) would enable light-induced activation of tongue motor units during spontaneous breathing. An AAV9 vector (pACAGW-ChR2-Venus-AAV9, 8.29E11vg) was injected into the posterior tongue in adult C57BL/6Jmice, targeting the genioglossus. After 12 weeks, mice were anesthetized and posterior tongue electromyographic (EMG) activity was recorded during spontaneous breathing. Photostimulation of the tongue was achieved using a high-powered 473 nm light emitting diode positioned near the injection site. Single pulse as well as pulse train stimulation paradigms of varied intensity and duration were performed. Light-evoked EMG responses increased with the intensity (1-way ANOVA on ranks, p=0.002) and duration (1-way ANOVA on ranks, p 2R01HD052682-11A1 (DDF, BJB), MBI Graduate Fellowship Award (MLS) This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.
Databáze: OpenAIRE
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