| Autor: |
R. Mohrlok, Britta Hagmeyer, Kai Fuchsberger, J. Weile, Andreas Moeller, Massimo Kubon, Martin Stelzle, Meike Moschallski, Christian Dorrer, Peter Rothacher, Karl-Heinz Boven |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
IFMBE Proceedings ISBN: 9783642038860 |
| Popis: |
We present a microfluidic chip that collects and accumulates bacteria from a sample by positive dielectrophoresis and subsequently lyses cells to retrieve DNA / RNA. Biological samples containing Escherichia coli are transferred to a suitable medium (low conductivity) by on-chip dialysis. This sample is pumped through a microchannel comprising an array of interdigitated electrodes at the bottom. An AC-voltage is applied to the electrodes resulting in an inhomogeneous electric field that extends into the channel and induces dielectrophoretic forces acting on the bacteria. Herringbone- mixer structures between the electrodes induce vortices in the flow and thus rotate the fluid volume from top to bottom. This assures that all bacteria are brought under the influence of the electric field and thus may be trapped. In addition, the herringbone structures induce further inhomogeneities in the electric field. Due to this combined effect, the system allows for the trapping of bacteria even at high flow rates which results in high throughput. Bacteria from several mL of sample are concentrated into 20 μL inside the chamber. This is monitored on-line by measuring the impedance between the electrodes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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