Antioxidant and cytoprotective effects of avocado oil and extract (Persea americanaMill) against rotenone using monkey kidney epithelial cells (Vero)
| Autor: | Francine Carla Cadoná, Alencar Kolinski Machado, Pâmela Jéssyca Hoss Longhi, Larissa Machado, Mathias Martins, Sergio Abreu Machado, Jovani Antônio Steffani, Nelzi Ferreira Queiroz Junior, Marco Aurélio Echart Montano |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Persea Antioxidant biology Health Toxicology and Mutagenesis medicine.medical_treatment food and beverages Rotenone Pharmacology Toxicology biology.organism_classification medicine.disease_cause Lipid peroxidation chemistry.chemical_compound chemistry medicine Viability assay Avocado Oil Oxidative stress |
| Zdroj: | Journal of Toxicology and Environmental Health, Part A. 84:875-890 |
| ISSN: | 1087-2620 1528-7394 |
| Popis: | Oxidative stress is known to be involved in development of numerous diseases including cardiovascular, respiratory, renal, kidney and cancer. Thus, investigations that mimic oxidative stress in vitro may play an important role to find new strategies to control oxidative stress and subsequent consequences are important. Rotenone, widely used as a pesticide has been used as a model to simulate oxidative stress. However, this chemical was found to produce several diseases. Therefore, the aim of this study was to investigate the antioxidant and cytoprotective effect of avocado (Persea americana Mill) extract and oil in monkey kidney epithelial cells (VERO) exposed to rotenone. VERO cells were exposed to IC50 of rotenone in conjunction with different concentrations of avocado extract and oil (ranging from 1 to 1000 µg/ml), for 24 hr. Subsequently, cell viability and oxidative metabolism were assessed. Data demonstrated that avocado extract and oil in the presence of rotenone increased cellular viability at all tested concentrations compared to cells exposed only to rotenone. In addition, extract and avocado oil exhibited antioxidant action as evidenced by decreased levels of reactive oxygen species (ROS), superoxide ion, and lipid peroxidation, generated by rotenone. Further, avocado extract and oil appeared to be safe, since these compounds did not affect cell viability and or generate oxidative stress. Therefore, avocado appears to display a promising antioxidant potential by decreasing oxidative stress. |
| Databáze: | OpenAIRE |
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