Effects of Nitric Oxide Synthase Inhibition on Cutaneous Vasodilation in Response to Acupuncture Stimulation in Humans
| Autor: | Kenichi Kimura, Hayato Takeuchi, Kuniko Yuri, Ikuro Wakayama |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Microdialysis
biology business.industry Vasodilation General Medicine Prilocaine Nitric oxide Nitric oxide synthase chemistry.chemical_compound Complementary and alternative medicine chemistry Anesthesia biology.protein medicine Reflex Acupuncture Axon reflex Neurology (clinical) business medicine.drug |
| Zdroj: | Acupuncture in Medicine. 31:74-80 |
| ISSN: | 1759-9873 0964-5284 |
| Popis: | Objectives The aim of the present study was to elucidate the mechanism of cutaneous vasodilation following acupuncture stimulation by investigating the roles of nitric oxide (NO) and axon reflex vasodilation. Methods The subjects were 17 healthy male volunteers. The role of NO was investigated by administering NG-nitro-L-arginine methyl ester hydrochloride (L-NAME, 20 mM), an NO synthase inhibitor or Ringer's solution (control site), via intradermal microdialysis (protocol 1; n=7). The role of axon reflex vasodilation by local sensory neurones was investigated by comparing vasodilation at sites treated with ‘eutectic mixture of local anaesthetics’ (EMLA) cream (2.5% lidocaine and 2.5% prilocaine) with untreated sites (control site) (protocol 2; n=10). After 5 min of baseline recording, acupuncture was applied to PC4 and a control site in proximity to PC4 for 10 min and scanning was performed for 60 min after acupuncture stimulation. Skin blood flow (SkBF) was evaluated by laser Doppler perfusion imaging. Cutaneous vascular conductance (CVC) was calculated from the ratio of SkBF to mean arterial blood pressure. Results In the first protocol, sites administered L-NAME showed significant reductions in CVC responses following acupuncture stimulation compared to control sites (administered Ringer's solution) (p0.05). Conclusions These data suggest that cutaneous vasodilation in response to acupuncture stimulation may not occur through an axon reflex as previously reported. Rather, NO mechanisms appear to contribute to the vasodilator response. |
| Databáze: | OpenAIRE |
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