Measurement of the permeability and resealing time constant of the electroporated mammalian cell membranes

Autor:	Vladimir L. Sukhorukov, Ryo Shirakashi, Ulrich Zimmermann, Ichiro Tanasawa
Rok vydání:	2004
Předmět:	Fluid Flow and Transfer Processes Membrane permeability medicine.diagnostic_test Chemistry Mechanical Engineering Electroporation Time constant Electrolyte Condensed Matter Physics Fluorescence Flow cytometry Membrane Permeability (electromagnetism) Biophysics medicine
Zdroj:	International Journal of Heat and Mass Transfer. 47:4517-4524
ISSN:	0017-9310
DOI:	10.1016/j.ijheatmasstransfer.2004.04.007
Popis:	In this study a new method is presented for measuring the transient permeability of mammalian cell membranes to sugar and electrolyte molecules based on the volumetric response of cells subjected to electroporation. The time constant of membrane resealing was determined independently by flow cytometry using a fluorescent dye as the reporter molecule. The volumetric and dye uptake data were analyzed with a model relating the cell volume changes to the solute transport across the reversibly permeabilized cell membrane. The experimental approach developed here might be useful for estimating the amount of electroinjected molecules, which are difficult to measure directly.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::42c1f4d10b69368146c700c925c1f044 https://doi.org/10.1016/j.ijheatmasstransfer.2004.04.007 Zobrazit plný text záznamu Full Text from ScienceDirect