Thyrotropin Receptor Expression in Graves’ Orbital Adipose/Connective Tissues: Potential Autoantigen in Graves’ Ophthalmopathy1
| Autor: | Rebecca S. Bahn, Christine Spitzweg, Armin E. Heufelder, Neena Natt, Werner Joba, Charyl M. Dutton |
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| Rok vydání: | 1998 |
| Předmět: |
endocrine system
Pathology medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism Graves' disease Clinical Biochemistry Adipose tissue Connective tissue Biology Biochemistry Thyrotropin receptor Graves' ophthalmopathy chemistry.chemical_compound Endocrinology Internal medicine Adipocyte medicine Fibroblast Biochemistry (medical) medicine.disease eye diseases medicine.anatomical_structure chemistry Connective tissue metabolism hormones hormone substitutes and hormone antagonists |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 83:998-1002 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.83.3.4676 |
| Popis: | It is acknowledged that the TSH receptor (TSHr) on thyroid follicular cells is the autoantigen involved in the hyperthyroidism of Graves’ disease. However, whether this receptor is expressed in extrathyroidal tissues, and whether it participates directly in the pathogenesis of Graves’ ophthalmopathy (GO) are unclear. We sought to detect the expression of TSHr messenger ribonucleic acid (mRNA) and protein in orbital adipose/connective tissue specimens and in human orbital preadipocyte fibroblast cultures using liquid hybridization analysis and immunohistochemical methods. We demonstrated intact and variant TSHr mRNA transcripts and TSHr-like immunoreactivity in orbital adipose/connective tissue specimens from patients with GO. In addition, TSHr-like immunoreactivity was detected in early passage GO preadipocyte fibroblast cultures that were shown to include some adipose cells. In contrast, neither TSHr mRNA nor protein was detected in normal orbital adipose/connective tissue specimens or in late passage GO... |
| Databáze: | OpenAIRE |
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