Gold Nanobipyramids as Second Near Infrared Optical Coherence Tomography Contrast Agents for in Vivo Multiplexing Studies
| Autor: | Adam de la Zerda, Peng Si, George W. Sledge, Edwin Yuan, Ziv Lautman, Ke Yuan, Stefanie S. Jeffrey, Saba Shevidi |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
medicine.diagnostic_test Mechanical Engineering Melanoma Sentinel lymph node Bioengineering General Chemistry Condensed Matter Physics medicine.disease Lymphatic system medicine.anatomical_structure Optical coherence tomography Microangiography medicine General Materials Science Molecular imaging Lymph node Preclinical imaging Biomedical engineering |
| Zdroj: | Nano Letters. 20:101-108 |
| ISSN: | 1530-6992 1530-6984 |
| Popis: | Developing contrast-enhanced optical coherence tomography (OCT) techniques is important for specific imaging of tissue lesions, molecular imaging, cell-tracking, and highly sensitive microangiography and lymphangiography. Multiplexed OCT imaging in the second near-infrared (NIR-II) window is highly desirable since it allows simultaneous imaging and tracking of multiple biological events in high resolution with deeper tissue penetration in vivo. Here we demonstrate that gold nanobipyramids can function as OCT multiplexing contrast agents, allowing high-resolution imaging of two separate lymphatic flows occurring simultaneously from different drainage basins into the same lymph node in a live mouse. Contrast-enhanced multiplexed lymphangiography of a melanoma tumor in vivo shows that the peritumoral lymph flow upstream of the tumor is unidirectional, and tumor is accessible to such flow. Whereas the lymphatic drainage coming out from the tumor is multidirectional. We also demonstrate real-time tracking of the contrast agents draining from a melanoma tumor specifically to the sentinel lymph node of the tumor and the three-dimensional distribution of the contrast agents in the lymph node. |
| Databáze: | OpenAIRE |
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