Fast-response fluorescent probe with favorable water solubility for highly sensitive imaging of endogenous tyrosinase in living cells and zebrafish model
| Autor: | Shuilin Wu, Guichun Yang, Xiaofeng Xia, Feiyi Wang, You Yu, Chao Ma, Jun Ren, Li Zheng, Junqi Nie, Qi Sun, Cuifen Lu |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Oxidase test Fluorophore Tyrosinase 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence In vitro 0104 chemical sciences chemistry.chemical_compound Enzyme chemistry In vivo Biophysics 0210 nano-technology Cytotoxicity |
| Zdroj: | Chinese Chemical Letters. 32:1785-1789 |
| ISSN: | 1001-8417 |
| DOI: | 10.1016/j.cclet.2020.12.053 |
| Popis: | Tyrosinase (TYR) is an important polyphenolic oxidase enzyme and usually regards as a biomarker of melanoma cancer. Highly effective tracking TYR activity in vivo will help to study the mechanism of TYR in living organisms and forecasts related diseases. In this study, we present a novel TYR-activatable fluorescent probe (CHMC-DOPA) for tracking TYR activity in vitro and in vivo. CHMC-DOPA is constructed by incorporating dopamine (DOPA) moiety into a fluorescent chloro-hydroxyl-merocyanine (CHMC) scaffold. Upon exposure to TYR, the dopamine unit in CHMC-DOPA is oxidized to a dopaquinone derivative, and an intramolecular photo-induced electron transfer (PET) process between CHMC fluorophore and o-dopaquinone will take place, the fluorescence of CHMC-DOPA is quenched rapidly. Therefore, the evaluation of TYR activity is established in terms of the relationship between fluorescence quenching efficiency and TYR activity. In our experiments, CHMC-DOPA shows various advantages, such as fast response (8⬰min), low concentration of TYR activation (0.5 U/mL), good water-solubility, as well as the lowest detection limit (0.003 U/mL) compared with previously reported works. Furthermore, CHMC-DOPA also exhibits excellent cell membrane permeability and low cytotoxicity, which is successfully used to monitor endogenous TYR activity in living cancer cells and zebrafish models. CHMC-DOPA performs well, and we anticipate that this newly designed novel platform will provide an alternative for high effective monitoring TYR activity in biosystems. |
| Databáze: | OpenAIRE |
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