Microsatellite loci for an endemic stingless bee Melipona seminigra merrillae (Apidae, Meliponini) from Amazon

Autor:	A. C. B. Sousa, Izaura Bezerra Francini, Danilo Augusto Sforça, Maria Imaculada Zucchi, Anete Pereira de Souza, C. G. Nunes-Silva, F. W. Cidade, Tatiana de Campos, Gislene Almeida Carvalho-Zilse
Rok vydání:	2009
Předmět:	Genetic diversity Apidae biology Stingless bee Amazon rainforest Zoology biology.organism_classification Loss of heterozygosity Botany Genetics Polymorphic Microsatellite Marker Microsatellite Melipona Ecology Evolution Behavior and Systematics
Zdroj:	Conservation Genetics Resources. 1:487-490
ISSN:	1877-7260 1877-7252
Popis:	Melipona seminigra merrillae is one of the main pollinators of Central Amazon native flora. These stingless bees are reared as economic alternative and for forest conservation purposes. Nine polymorphic microsatellite markers were characterized in 44 genotypes of M. seminigra merrillae from the Meliponary of the Instituto Nacional de Pesquisas da Amazonia, INPA (3° 05.838′S 059° 59.103′W), Manaus, Brazil. The number of alleles observed for each locus ranged from 3 to 8, with an average of 3.7 alleles per locus. The polymorphism information content (PIC) varied between 0.21 and 0.81 (average 0.50) and the discriminating power (D) ranged from 0.59 to 0.98 (average 0.77). The observed heterozygosity (H o) and the expected heterozygosity (H e) were 0.04–0.68 and 0.19–0.84, respectively. A cross-amplification test in three Melipona species suggested potential transferability of these microsatellites. These microsatellite markers should be useful for characterizing natural populations and specie conservation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::429d4885e48d1f373cb71cdf568f3cb3 https://doi.org/10.1007/s12686-009-9113-9 Zobrazit plný text záznamu