Alteration of sarcoplasmic reticulum Ca2+ ATPase expression in lower limb ischemia caused by atherosclerosis obliterans
| Autor: | Tamás Oláh, László Csernoch, Ilona Kovács, I Ioannis, B Molnár, A Gomba-Tóth, Zs Cs Tóth, János Fodor, Ernő Zádor |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene isoform medicine.medical_specialty SERCA biology Cholesterol ATPase Endoplasmic reticulum Lumen (anatomy) Skeletal muscle Anatomy 03 medical and health sciences Cytosol chemistry.chemical_compound 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Physiology (medical) Internal medicine medicine biology.protein |
| Zdroj: | Physiology International. 104:183-192 |
| ISSN: | 2498-602X |
| DOI: | 10.1556/2060.104.2017.2.1 |
| Popis: | Atherosclerosis is a disease caused by a build-up of fatty plaques and cholesterol in the arteries. The lumen of the vessels is obliterated resulting in restricted blood supply to tissues. In ischemic conditions, the cytosolic Ca2+ level of skeletal muscle may increase, indicating the alteration of Ca2+ removal mechanisms. Ca2+ is transported from cytosol into the sarcoplasmic reticulum by Ca2+ ATPase (SERCA), with its 1a isoform expressed in adult, while its 1b isoform in neonatal and regenerating fast-twitch skeletal muscle. To investigate the role of these isoforms in ischemic skeletal muscle, biopsies from musculus biceps femoris of patients who underwent amputation due to atherosclerosis were examined. Samples were removed from the visibly healthy and hypoxia-affected tissue. Significantly increased SERCA1a expression was detected under the ischemic conditions (246 ± 69%; p p > 0.05), whereas SERCA2a did not change. In addition, in primary cultures derived from hypoxia-affected tissue, the diameter and fusion index of myotubes were significantly increased (30 ± 1.6 µm vs. 41 ± 2.4 µm and 31 ± 4% vs. 45 ± 3%; p |
| Databáze: | OpenAIRE |
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