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Background: Infertility is an important health problem affecting the quality of life accompanied by significant emotional and psychological consequences. Infertile couples living with emotional disturbances face challenges with less success of infertility treatment. Purposes: This study aimed to investigate the quality of life of infertile couples who seek In-Vitro Fertilizations (IVFs) by gender in Gaza city, Palestinian. Methods and materials: A cross sectional study was conducted, from Feb 2018 to Nov 2018, among 383 infertile couples selected through convenient sampling. A valid and reliable Arabic version of the fertility quality of life questionnaire (FertiQoL) was used in data collection. One way ANOVA and independent t-test was applied to compare between males and females. P value less than 0.05% was considered statistical significance. Results: The mean age of males and females was 34.54±8.54 and 29.28±6.71 years respectively. More than half of them had university degree (64% and 60% respectively). The mean duration of infertility of couples was 5.66±3.54 years. The mean males' scores of FertiQoL and its subscales (emotion, mind/body, social, core, tolerability and treatment) were significantly higher than females' scores (p |