Expression of the troponin complex genes: transcriptional coactivation during myoblast differentiation and independent control in heart and skeletal muscles

Autor:	Charles P. Emerson, P C Maisonpierre, E A Bucher, Stephen F. Konieczny
Rok vydání:	1988
Předmět:	Regulation of gene expression Troponin T macromolecular substances Cell Biology Biology musculoskeletal system Troponin Molecular biology Troponin C Troponin complex Troponin I Gene expression biology.protein Myocyte Molecular Biology
Zdroj:	Molecular and Cellular Biology. 8:4134-4142
ISSN:	1098-5549 0270-7306
Popis:	We compared the developmental regulation of the three troponin genes that encode the proteins of the Ca2+ regulatory complex in striated muscles of the Japanese quail. Nuclear run-on transcription and RNA protection analyses showed that the fast skeletal troponin I, the fast skeletal troponin T, and the slow skeletal-cardiac troponin C genes were transcriptionally coactivated and that transcripts rapidly accumulated within 6 to 12 h after the initiation of myoblast differentiation. The fast-isoform mRNAs of troponin I and troponin T were coexpressed at similar levels in different skeletal muscles, whereas the slow-cardiac troponin C mRNA varied independently and was the only one of these genes expressed in embryonic and adult heart. We conclude that these troponin genes are transcriptionally coactivated during skeletal myoblast differentiation, indicating that their transcription is under precise temporal control. However, this troponin C gene is regulated independently is specialized striated muscles.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4278fd01a1a324a5e1d87eb9e9052828 https://doi.org/10.1128/mcb.8.10.4134 Zobrazit plný text záznamu