Rapid detection of serum free light chains by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
| Autor: | Zelal Zuhal Kaya, Muhittin Serdar, Fehime Aksungar, Meltem Kilercik, Mustafa Serteser, Ahmet Tarik Baykal |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | European Journal of Mass Spectrometry. 29:132-140 |
| ISSN: | 1751-6838 1469-0667 |
| DOI: | 10.1177/14690667231153616 |
| Popis: | Introduction: Serum free light chain (FLC) measurements are increasingly prominent for patients with plasma cell disorders (PCDs) in screening, prognostic stratification, and monitoring therapy responses. Objectives: We aimed to develop a sensitive, reliable, and accurate method for diagnosing PCDs that can notably decrease the time and cost of current methods. Methods: Here, we present a novel approach for FLC measurement using immunoenrichment on micro-affinity chromatography in combination with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) detection. In this study, serum free kappa (κ) and free lambda (λ) light chain (LC) levels in the serum of 105 patients were compared between the nephelometric serum FLC quantification and MALDI-TOF MS detection. Results: Cohen's kappa coefficient between the MALDI-TOF MS-based method and the FLC assay revealed an almost perfect agreement in the case of normal (negative) results (κ = 0.92; 95% confidence interval (CI): 0.837 to 0.968) and a good agreement in the case of increased (positive) results (κ = 0.76; 95% CI: 0.608 to 0.870). In Spearman's correlation analysis, the best correlation was found between serum free κ/λ ratios (r = 0.628, 0.496 to 0.732; p |
| Databáze: | OpenAIRE |
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