| Popis: |
The following chlorinated hydrocarbons, CCl4, Cl2CH2, chloroform, 1,1,2-trichloroethane, 1,1-dichloroethylene, 1,1,2-trichloroethylene, and 1,1,2,2-tetrachloroethylene, were administered 1 day before testing. The test consisted of a retrograde intrabiliary injection of [3H]inulin followed by a 6-min period of occlusion, to hold the [3H]inulin solution in the biliary tree, and then collecting and analyzing each drop of bile. The content of [3H]inulin in the drops from the distal portion of the biliary tree was diluted by increased excretion of “bile duct-pancreatic fluid” into the duct system during the 6-min occlusion period. An increase in bile duct-pancreatic fluid flow in chlorinated hydrocarbon-treated rats was directly demonstrated by cannulating the common bile duct at the proximal bifurcation (to collect hepatic bile) and distally at the duodenum (to collect bile duct-pancreatic fluid). The results showed that the chlorinated hydrocarbons produced an increase in bile duct-pancreatic fluid flow. The increase in this distal fluid flow was not correlated to a rise in SGPT activity or extent of liver cell necrosis because both 1,1-dichloroethylene, which produced high SGPT activity and necrosis, and chloroform, low SGPT and no necrosis, increased bile duct-pancreatic fluid flow to a similar extent. |
