PCR–RFLP and species-specific PCR efficiency for the identification of adulteries in meat and meat products
| Autor: | Héla Gargouri, Hassen Hadj Kacem, Nizar Moalla |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Enzymatic digestion 030309 nutrition & dietetics food and beverages Species detection 04 agricultural and veterinary sciences General Chemistry Biology 040401 food science Biochemistry Industrial and Manufacturing Engineering 03 medical and health sciences 0404 agricultural biotechnology Mitochondrial cytochrome Multiplex Food science Donkey Restriction fragment length polymorphism Triplex PCR Daily routine Food Science Biotechnology |
| Zdroj: | European Food Research and Technology. 247:2183-2192 |
| ISSN: | 1438-2385 1438-2377 |
| Popis: | The traceability of meat origin has become a necessity to safeguard consumer's confidence in commercial meat products. Our study recommends a meat species detection using qualitative approach based on PCR–RFLP and species-specific primers PCR. Here, we targeted a 359 bp fragment of the mitochondrial cytochrome b gene amplified by PCR using universal primers followed by three enzymatic digestions. Seven animal species including dromedary, rabbit, goat, turkey, rat, donkey and pork, have been efficiently detected in pure and mixed samples. The combination of PCR–RFLP and triplex PCR assays offers, in addition, the identification of chicken, dog and cat species in meat. In conclusion, by the mean of PCR-based techniques using universal primers followed by enzymatic digestion and multiplex primer-specific approach, we developed an extensible protocol by which we identified 10 animal species that could be integrated in meat analysis daily routine. |
| Databáze: | OpenAIRE |
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