Airborne particulate matter, hydroxyl radical production and cellular toxicity

Autor:	Peter L. Gutierrez, M. Alaghmand
Rok vydání:	2011
Předmět:	Diesel exhaust Chemistry Health Toxicology and Mutagenesis Radical Clone (cell biology) Particulates medicine.disease_cause Pollution chemistry.chemical_compound Cell culture Environmental chemistry Toxicity medicine Environmental Chemistry Hydroxyl radical Oxidative stress
Zdroj:	Toxicological & Environmental Chemistry. 93:955-973
ISSN:	1029-0486 0277-2248
DOI:	10.1080/02772248.2011.571810
Popis:	Studies suggest that oxidative stress is a cause for the observed pathology in lung after exposures to air pollution. The aim of this study was to examine the effects of different airborne particulate matter (PM) on the survival of human bronchial epithelial cells immortalized with adenovirus-12 SV40 hybrid virus clone 2b (BEAS-2B cells, S6 subclone; BEAS). This was achieved by correlating cell survival directly with the flux of hydroxyl radicals (•OH) generated in a mixture of cells and particulates. A relatively new and highly sensitive fluorescence-based method was used to detect and quantify •OH. Two different toxicological methods were employed to test BEAS-2B cell survival in the presence of various types of airborne PM. Low •OH production was observed when this cell line was exposed to diesel particulate matter resulting in moderate toxicity. Urban dust (UD) which produced similar •OH flux in the presence of cells, resulted in protection from phosphate buffer saline toxicity, a required control. Am...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4260a33df601371788d338c62374a51c https://doi.org/10.1080/02772248.2011.571810 Zobrazit plný text záznamu