| Popis: |
Ipfencarbazone is a herbicide of the tetrazolinone class, and is believed to be an inhibitor of verylong chain fatty acids (VLCFAs), which control cell division in weeds. The objective of this study was todevelop and validate an official analytical method for ipfencarbazone determination in agricultural products.The ipfencarbazone residues in agricultural products were extracted with acetone, partitioned with n-hexane,and then purified through silica SPE cartridge. Finally, the analyte was quantified by gas chromatograph-electron capture detector (GC-ECD) and confirmed with gas chromatograph/mass spectrometer(GC/MS). Thelinear range of ipfencarbazone was 0.01 to 1.0 mg/L with the coefficient of determination (r 2 ) of 0.9999. Thelimit of detection (LOD) and quantification (LOQ) was 0.003 and 0.01 mg/kg, respectively. In addition,average recoveries of ipfencarbazone ranged from 80.6% to 112.3% at the different concentration levels LOQ,10LOQ and 50LOQ, while the relative standard deviation was 2.2-8.6%. All values were consistent with thecriteria ranges requested in the CODEX guidelines. Furthermore, and inter-laboratory study was conducted tovalidate the method. This proposed method for determination of ipfencarbazone residues in agriculturalproducts can be used as an official analytical method.Key words Ipfencarbazone, GC-ECD, GC/MS, Herbicide, Maximum Residue Limits |
