Biosensoric detection of the cysteine sulphoxide alliin
| Autor: | Michael Keusgen, Michael J. Schöning, Ingo Krest, Martina Jünger |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Chromatography
biology Chemistry Metals and Alloys Alliin Condensed Matter Physics Allium sativum biology.organism_classification High-performance liquid chromatography Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Biochemistry Alliinase Materials Chemistry biology.protein Potentiometric sensor Allium Electrical and Electronic Engineering Instrumentation Biosensor Cysteine |
| Zdroj: | Sensors and Actuators B: Chemical. 95:297-302 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/s0925-4005(03)00427-1 |
| Popis: | Garlic ( Allium sativum L.) and related species of the Alliaceae family are known for their cancer-protecting and antiatherosclerotic potential. The bioactivity of these plants is related to organo-sulphur compounds, mainly alliin. In the present investigation, an alliin-specific biosensor exploiting immobilised alliinase (EC 4.4.1.4) has been developed. Enzymatically formed ammonia was detected by a potentiometric sensor based on an ammonia electrode or a pH-sensitive electrolyte/insulator/semiconductor (EIS) layer structure made of Al/p-Si/SiO 2 /Si 3 N 4 . It could be demonstrated with both methods that this biosensoric approach yielded results comparable to sensitivities obtained by HPLC. Alliin concentrations down to 6×10 −6 M could be quantified. The EIS biosensor showed also sensitivity to further cysteine sulphoxides, like methiin, ethiin and propiin, which are common for wild Allium species. Besides the analysis of garlic and garlic-containing preparations, like herbal remedies, this biosensor can be additionally used for breeding research and screening purposes of potential medicinal plants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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