Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton
| Autor: | Patricia Occhipinti, Amy S. Gladfelter, Maximilian S. Jentzsch, Andrew A. Bridges, Patrick W. Oakes |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
macromolecular substances Cell Biology Biology Curvature Septin Cell biology Cell membrane 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Membrane curvature medicine Signal transduction Lipid bilayer Cytoskeleton 030217 neurology & neurosurgery Septin cytoskeleton |
| Zdroj: | Journal of Cell Biology. 213:23-32 |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.201512029 |
| Popis: | Cells change shape in response to diverse environmental and developmental conditions, creating topologies with micron-scale features. Although individual proteins can sense nanometer-scale membrane curvature, it is unclear if a cell could also use nanometer-scale components to sense micron-scale contours, such as the cytokinetic furrow and base of neuronal branches. Septins are filament-forming proteins that serve as signaling platforms and are frequently associated with areas of the plasma membrane where there is micron-scale curvature, including the cytokinetic furrow and the base of cell protrusions. We report here that fungal and human septins are able to distinguish between different degrees of micron-scale curvature in cells. By preparing supported lipid bilayers on beads of different curvature, we reconstitute and measure the intrinsic septin curvature preference. We conclude that micron-scale curvature recognition is a fundamental property of the septin cytoskeleton that provides the cell with a mechanism to know its local shape. |
| Databáze: | OpenAIRE |
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