Synthesis of a grapevine phytoalexin in transgenic tomatoes (Lycopersicon esculentumMill.) conditions resistance againstPhytophthora infestans
| Autor: | Jürgen Thomzik, Ruediger Hain, Ronald Helmut Stöcker, Klaus Stenzel, P H Schreier, D.J Stahl |
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| Rok vydání: | 1997 |
| Předmět: |
chemistry.chemical_classification
biology Phytoalexin fungi Alternaria solani food and beverages Plant Science Agrobacterium tumefaciens biology.organism_classification Lycopersicon Microbiology Elicitor Trihydroxystilbene synthase chemistry Phytophthora infestans Botany Genetics biology.protein Genetically modified tomato |
| Zdroj: | Physiological and Molecular Plant Pathology. 51:265-278 |
| ISSN: | 0885-5765 |
| DOI: | 10.1006/pmpp.1997.0123 |
| Popis: | Stilbene synthase occurs in several plant species and synthesizes the stilbene phytoalexin trans-resveratrol using substrates commonly present in plants. The formation of stilbenes is considered to be a component of general defence mechanisms against pathogens in plants. Two genes from the grapevine ( Vitis vinifera ) coding for stilbene synthase were transferred to tomato (cv. Vollendung) by means of Agrobacterium tumefaciens. Southern blot and Northern blot analyses demonstrated that both genes were stably integrated into the tomato genome and were expressed in transgenic regenerants and their progeny after fungal infection, wounding, induction with elicitor, and u.v. irradiation. The accumulation of the phytoalexin trans-resveratrol, the product of stilbene synthase, was detectable shortly after fungal inoculation, resulting in a significant increase in the resistance of transgenic tomato to Phytophthora infestans. A similar accumulation of resveratrol occurred after inoculation with Botrytis cinerea and Alternaria solani, but no significant increase in resistance was observed in transgenic tomato plants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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