| Autor: |
D. Simpson, R. Grant, D. A. Smail, N. Bain, T. S. Hastings |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Aquaculture. 240:29-38 |
| ISSN: |
0044-8486 |
| DOI: |
10.1016/j.aquaculture.2004.05.045 |
| Popis: |
Following the infectious salmon anaemia outbreak in Scotland in May 1998, there was a strong interest in evaluating alternatives to sodium hypochlorite for the disinfection of fish farming equipment. Because no information was available, a programme of laboratory testing of several disinfectants against cultured ISA virus was commenced. Cultured ISA virus was grown on permissive cell lines and a stock stored at −80 °C. Disinfectant assays were set up using a procedure similar to that recommended for viruses of higher animals under the UK Animal Health Act (1981). Dilutions of disinfectant at the manufacturer's recommended dose were made in W.H.O. hard water at 4 °C and ISA virus was added for 5 min exposure. Neutralizer was added to inactivate the disinfectant and the samples were dialysed. Residual and starting virus was titrated on SHK-1 cells using a focus-forming assay (FFA). Briefly, cell layers were fixed with acetone, incubated with diluted ISA virus monoclonal antibody 3H6F8, then incubated with diluted goat anti-mouse Ig phosphatase conjugate and bound conjugate visualised with fast red TR/napthol-AS MX stain. Stained foci were counted. Virucidal reductions in titre of >4 log 10 focus forming units (ffu)/ml were obtained using the six types of virucide. A virus titre reduction of log 10 4 is the approved disinfectant inactivation standard in the UK according to the current DEFRA guideline protocol for disinfectant testing for veterinary viruses. According to this definition six branded disinfectants were found effective for the disinfection of fish farming equipment or fish ova at the manufacturer's recommended dose for 5 min duration. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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