| Autor: |
Timothy C. Kenny, Artem Khan, Yeeun Son, Lishu Yue, Søren Heissel, Eric R. Gamazon, Hanan Alwaseem, Richard Hite, Kıvanç Birsoy |
| Rok vydání: |
2022 |
| DOI: |
10.1101/2022.10.05.509986 |
| Popis: |
Genome-wide association studies (GWAS) of serum metabolites have the potential to uncover genes that influence human metabolism. Here, we combined an integrative genetic analysis associating serum metabolites to membrane transporters with a coessentiality map of metabolic genes. This analysis revealed a connection between feline leukemia virus subgroup C cellular receptor 1 (FLVCR1) - a plasma membrane protein - and phosphocholine, a downstream metabolite of choline metabolism. Loss of FLVCR1 in human cells and in mice strongly impairs choline metabolism due to a block in choline import. Consistently, CRISPR-based genetic screens identified several components of the membrane phospholipid machinery as synthetic lethal with FLVCR1 loss. Finally, cells lacking FLVCR1 exhibit mitochondrial defects and upregulate the integrated stress response (ISR) through heme regulated inhibitors kinase (HRI). Altogether, these findings identify FLVCR1 as a universal mediator of choline transport in mammals and provide a platform to discover substrates for unknown metabolite transporters. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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