| Autor: |
Adrian Bowyer, John Hubble, Ian Benzeval, Shuang Yu |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
Journal of Membrane Science. 339:138-142 |
| ISSN: |
0376-7388 |
| DOI: |
10.1016/j.memsci.2009.04.041 |
| Popis: |
A novel method for the preparation of a composite pore-filled membrane based on self-assembled hydrogels is described. Responsive pore-filled membranes based on mixtures of dextran (molecular weight 6000) and the glucose binding lectin Concanavalin A (ConA) were prepared. The transport properties of these membranes were characterised using a twin chamber diffusion cell with Cibacron blue as a tracer solute. As the ConA molecules introduce affinity cross-links into the gel structure by binding to terminal glucose moieties on the dextran chains the mesh size increased as the physical cross-link density of the hydrogel decreased with increasing competing glucose concentration. This increased the gel-phase diffusion coefficient for the tracer with results obtained for the effect of glucose on transport rates showing a linear increase up to a concentration of 40 mM. Further increases in d -glucose concentration lead to a subsequent decrease in transport rate which is attributed to the effects of gel swelling increasing the diffusion path length. Control experiments conducted with l -glucose, which does not bind to ConA, showed no increase in transport rate confirming a specific competition effect. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect