Development and Validation of a Reversed Phase HPLC Method for Simultaneous Determination of Curcumin and Piperine in Human Plasma for Application in Clinical Pharmacological Studies
| Autor: | Sharmila Mishra, Geoffrey Edwards, Prerana Sethi, Virendra K. Dua, Rajeev Jain, Subhendra Mohanty |
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| Rok vydání: | 2009 |
| Předmět: |
Detection limit
Monolithic HPLC column Chromatography Chemistry Clinical Biochemistry Pharmaceutical Science Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Pharmacokinetics Piperine Curcumin Quantitative analysis (chemistry) |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 32:2961-2974 |
| ISSN: | 1520-572X 1082-6076 |
| Popis: | A sensitive and specific HPLC method was developed for the simultaneous determination of curcumin and piperine in human plasma in view of the potential therapeutic application of curcumin and piperine in various diseases. The HPLC method consisted of isocratic elution with acetonitrile-methanol-trifluoroacetic acid-water (17.6:35.3:0.1:47.0, v/v/v/v) with a flow rate of 2.5 mL min−1 on a Chromolith® SpeedROD RP-18 (50 × 4.6 mm) column at an ambient temperature. Ultraviolet detection was performed in programe mode at 415 nm for curcumin, 335 nm for piperine, and 280 nm for β-17-estradiol acetate (internal standard). Curcumin, piperine, and internal standard were extracted from plasma using ethyl acetate-propanol (9:1 v/v). Mean extraction recoveries for curcumin, piperine, and internal standard were 91.3, 91.4, and 92.9%, respectively. The assay was linear over the therapeutic concentration range (10–500 ng mL−1) for both drugs with correlation coefficients of r2 > 0.99. Limit of detection and lim... |
| Databáze: | OpenAIRE |
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