Identification of functional flavonol synthase genes from fragrant wild cyclamen (Cyclamen purpurascens)
| Autor: | Satoshi Kitamura, Riho Mikami, Yusuke Akita, Hiroshi Ishizaka |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mutant Plant Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Flavonols Botany Flavonol synthase Arabidopsis thaliana Gene Cyclamen purpurascens chemistry.chemical_classification fungi food and beverages biology.organism_classification 030104 developmental biology chemistry Anthocyanin biology.protein Agronomy and Crop Science Cyclamen 010606 plant biology & botany Biotechnology |
| Zdroj: | Journal of Plant Biochemistry and Biotechnology. |
| ISSN: | 0974-1275 0971-7811 |
| DOI: | 10.1007/s13562-017-0423-9 |
| Popis: | Cyclamen purpurascens is considered suitable for horticultural breeding of cyclamens because it has an attractive fragrance that is not found in other wild species. To improve the commercial value of cyclamen flowers, this fragrance has been introduced into ornamental cultivars. However, variation in flower color is somewhat limited in these cultivars, and therefore understanding the genetic networks of flower coloration in C. purpurascens is required. We previously isolated DNA fragments of anthocyanin biosynthetic genes from C. purpurascens, broadening our understanding of the biosynthetic pathway of flavonols, which are co-pigments in flower coloration. In this study, we isolated complete open reading frames of flavonol synthase genes from C. purpurascens (CpurFLS1 and CpurFLS2) and analyzed the in planta functions of the genes by molecular complementation assay using the fls mutant of Arabidopsis thaliana. Expression patterns in several organs of C. purpurascens were also determined. The results strongly suggest that the CpurFLS genes participate in flavonol synthesis. We discuss the involvement of these two FLSs in flower coloration in C. purpurascens. |
| Databáze: | OpenAIRE |
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