Cellular damage, including wounding, drives C. elegans stress-induced sleep
| Autor: | Desiree L Goetting, Rony Soto, Richard Mansfield, Cheryl Van Buskirk |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Nematode caenorhabditis elegans Stress induced AMPK Biology Sleep in non-human animals Cell biology 03 medical and health sciences Cellular and Molecular Neuroscience 030104 developmental biology 0302 clinical medicine Sensation Genetics Sleep pressure Protein kinase A 030217 neurology & neurosurgery Ultraviolet light exposure |
| Zdroj: | Journal of Neurogenetics. 34:430-439 |
| ISSN: | 1563-5260 0167-7063 |
| Popis: | Across animal phyla, sleep is associated with increased cellular repair, suggesting that cellular damage may be a core component of sleep pressure. In support of this notion, sleep in the nematode Caenorhabditis elegans can be triggered by damaging conditions, including noxious heat, high salt, and ultraviolet light exposure. It is not clear, however, whether this stress-induced sleep (SIS) is a direct consequence of cellular damage, or of a resulting energy deficit, or whether it is triggered simply by the sensation of noxious conditions. Here, we show that thermosensation is dispensable for heat-induced sleep, that osmosensation is dispensable for salt-induced sleep, and that wounding is also a sleep trigger, together indicating that SIS is not triggered by sensation of noxious environments. We present evidence that genetic variation in cellular repair pathways impacts sleep amount, and that SIS involves systemic monitoring of cellular damage. We show that the low-energy sensor AMP-activated protein kinase (AMPK) is not required for SIS, suggesting that energy deficit is not the primary sleep trigger. Instead, AMPK-deficient animals display enhanced SIS responses, and pharmacological activation of AMPK reduces SIS, suggesting that ATP-dependent repair of cellular damage mitigates sleep pressure. |
| Databáze: | OpenAIRE |
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