Molecular Detection of Anaplasma phagocytophilum from Larvae of Haemaphysalis longicornis in Ibaraki, Japan
| Autor: | Hisashi Inokuma, Yuichi Fukui |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Sequence analysis 030106 microbiology General Medicine Amplicon Ribosomal RNA Biology bacterial infections and mycoses 16S ribosomal RNA Haemaphysalis biology.organism_classification medicine.disease Virology Anaplasma phagocytophilum 03 medical and health sciences 0302 clinical medicine Infectious Diseases parasitic diseases medicine 030212 general & internal medicine Anaplasmosis Haemaphysalis longicornis |
| Zdroj: | Japanese Journal of Infectious Diseases. 72:423-425 |
| ISSN: | 1884-2836 1344-6304 |
| Popis: | We collected 1,084 ticks by flagging vegetation in Tsukuba and Moriya (Ibaraki, Japan), where several cases of canine granulocytic anaplasmosis were reported. The DNA of the collected ticks was molecularly examined for infection with the family Anaplasmataceae. Twenty-six positive samples of Anaplasmataceae-specific PCR of partial 16S rRNA gene were subjected to semi-nested PCR, covering the divergent regions, the gene, and sequence analysis. Anaplasma phagocytophilum was detected in 3 pools of Haemaphysalis longicornis larvae and A. bovis from a H. flava male. Sequences of both amplicons had high homologies to those from dogs in our previous studies in Ibaraki. These results suggest that Haemaphysalis ticks are candidate vectors of A. phagocytophilum and A. bovis in Ibaraki, Japan. |
| Databáze: | OpenAIRE |
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