The time-dependent variations of zebrafish intestine and gill after polyethylene microplastics exposure

Autor: Ying-Hao Xue, Xin-Li Wen, Zhi-Yu Xu, Liang-Shan Feng, Tuo Jin, Zhan-Xiang Sun, Feng-Yan Zhao
Rok vydání: 2021
Předmět:
Zdroj: Ecotoxicology. 30:1997-2010
ISSN: 1573-3017
0963-9292
DOI: 10.1007/s10646-021-02469-4
Popis: Microplastics (MPs) are common environmental contaminants that present a growing health concern due to their increasing presence in aquatic and human systems. However, the mechanisms behind MP effects on organisms are unclear. In this study, zebrafish (Danio rerio) were used as an in vivo model to investigate the potential risks and molecular mechanisms of the toxic effects of polyethylene MPs (45–53 μm). In the zebrafish intestine, 6, 5, and 186 genes showed differential expression after MP treatment for 1, 5, and 10 days, respectively. In the gills, 318, 92, and 484 genes showed differential expression after MP treatment for 1, 5, and 10 days, respectively. In both the intestine and the gills, Gene Ontology (GO) annotation showed that the main enriched terms were biological regulation, cellular process, metabolic process, cellular anatomical entity, and binding. KEGG enrichment analysis on DEGs revealed that the dominant pathways were carbohydrate metabolism and lipid metabolism, which were strongly influenced by MPs in the intestine. The dominant pathways in the gills were immune and lipid metabolism. The respiratory rate of gills, the activity of SOD and GSH in the intestine significantly increased after exposure to MPs compared with the control (p
Databáze: OpenAIRE
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