Isolation, Phenotypic and Molecular Identification of Actinomycetes From Soil and Evaluation of Their Efficiency in Control of the Pathogen Botrytis cinerea Caused Gray Rot Disease on Eggplant

Autor: Abdullah Abdulkareem Hassan, Abdullah Fadhil Mahmoud
Rok vydání: 2022
Předmět:
Zdroj: IOP Conference Series: Earth and Environmental Science. 1060:012108
ISSN: 1755-1315
1755-1307
DOI: 10.1088/1755-1315/1060/1/012108
Popis: The current study included the isolation of 29 isolates of Actinomycetes from soil samples that collected from different regions of Salah Al-Din Governorate, including districts (Samarra, Tikrit, Balad, Sharqat, Tuz and Ishaqi). The results of the antagonism of these isolates against the pathogenic fungus Botrytis cinerea showed the superiority of isolates S2, S5, Sh3, Sh1 and T2 on other isolates, the inhibition zone of the pathogenic fungus was 2.4, 2.2, 2.2, 2.1 and 1.9 cm, respectively. These isolates were identified using microscopic and biochemical tests, as well as their diagnosis molecularly to the species level using the nucleotide sequence analysis technique of 16S rRNA gene. All isolates belonged to the genus Streptomyces sp., the similarity percentage of the isolates ranged from (99.47-99.62%) with the Streptomyces spp. recorded in the database of the World GenBank. The above isolates were identified as Streptomyces kanamyceticus, S.hygroscopicus, S.achromogenes, S.atratus and S.griseus, respectively, and registered in the database of the World GenBank in NCBI. The filtrates of Streptomyces sp. grown in various media included; Glycerol Tyrosine Medium, Glycerol Yeast Extract Medium, Glycerol Aspargine Medium, Starch-Minerals Medium and Starch Peptone Yeast Extract Medium against pathogenic fungus B. cinerea, the results showed that the S. kanamyceticus filtrate recorded the highest inhibition rate in all media except Starch Mineral medium, which reached 8.13 and 8.66, 4.73, 4.46 and 5.46 mm, respectively. The effectiveness of some active substances with antifungal activity produced from the Streptomyces spp. was estimated, which included the antibiotic Fungichromin, chitinase and beta-glucanase, the results showed the production of fangichromin from S. kanamyceticus, S. hygroscopicus, and S.achromogenes only, which reched to 6.76, 6.66 and 5.4 μg/ml, respectively, on the othe hand, all isolates recorded the activity of chitinase and beta-glucanase, the highest activities were 0.895 and 1.254 units/ml in S. kanamyceticus, respectively.
Databáze: OpenAIRE
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