Effects of Anidulafungin and Voriconazole, Singly and in Combination, on Cytokine/Chemokine Production by Human Monocyte-Derived Macrophages Infected with Candida glabrata or Activated by Lipopolysaccharide
| Autor: | Phyllis B. Michelsen, David A. Lawrence, William J. Ritz, Cynthia J. Carlyn, Raymond P. Smith, Nancy Andersen, Lawrence H. Bopp, Aldona L. Baltch |
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| Rok vydání: | 2012 |
| Předmět: |
Chemokine
Lipopolysaccharide medicine.medical_treatment macromolecular substances Biology Microbiology chemistry.chemical_compound Drug Discovery medicine Pharmacology (medical) Severe sepsis Pharmacology Voriconazole Candida glabrata General Medicine bacterial infections and mycoses biology.organism_classification Infectious Diseases Cytokine Oncology chemistry Monocyte-Derived Macrophages Immunology biology.protein Anidulafungin medicine.drug |
| Zdroj: | Chemotherapy. 58:146-151 |
| ISSN: | 1421-9794 0009-3157 |
| Popis: | Background:Candida glabrata causes infections associated with severe sepsis, production of high concentrations of cytokines/chemokines, and high mortality. This study describes the effects of anidulafungin (ANF) and voriconazole (VRC), singly and in combination, on the production of eight cytokines/chemokines by human monocyte-derived macrophages (MDM) infected with C. glabrata or activated by lipopolysaccharide (LPS). Methods: MDM monolayers were established, infected with C. glabrata or activated with LPS, and then treated with high or low concentrations of ANF, VRC, or both. Cytokine/chemokine levels in MDM supernatants were determined. Results: Levels of cytokines/chemokines were significantly elevated in supernatants of infected or LPS-activated MDM. Except for interleukin-10, all significant decreases in cytokine/chemokine concentrations (p < 0.01) occurred in supernatants of infected MDM treated with high concentrations of ANF or ANF + VRC. Conclusions: Decreases in cytokine/chemokine levels in supernatants of infected MDM treated with high concentrations of ANF or ANF + VRC suggest that similar treatment could improve survival in patients with severe, invasive C. glabrata infections and markedly elevated levels of serum cytokines/chemokines. |
| Databáze: | OpenAIRE |
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